Technical note: an improved method to quantify nonesterified fatty acids in bovine plasma.

Tierärztliche Hochschule Hannover Investigation of antioxidative capacity in bovine seminal plasma– Effects of Omega-3 fatty acids INAUGURAL – DISSERTATION

P-1: Addition of Omega-3 Unsaturated Fatty Acids in Sperm Extender Does Not Improve The Quality of Chilled Bovine Sperm

Background: The importance of polyunsaturated fatty acids (PUFAs) on chilled sperm quality is under investigation. Addition of fatty acids to the diet can influence sperm quality and male fertilizing capacity. The aim of this study was to investigate if the addition of n-3 PUFAs improves quality of bovine chilled sperm. Materials and Methods: In order to do experiment, 5 proven bulls were rando...

Quantitative extraction and determination of nonesterified fatty acids in plasma.

A method is described for the determination of nonesterified fatty acids in plasma. Extraction is at least 98% efficient, and losses during subsequent stages are corrected for by the use of an internal radioactive standard. The method is suitable for reference purposes rather than for routine determinations. Higher values are obtained than by other methods of analysis, and it is suggested that ...

The Association of Omentin Gene Expression in Visceral and Subcutaneous Adipose Tissues with Plasma Fatty Acids Profile and Dietary Fatty Acids

Introduction: Omentin, an adipokine, with anti-inflammatory effects reduces insulin resistance, and can hence, play an important role in prevention of cardiovascular disease and diabetes. The present study aimed to investigate the association of plasma and dietary fatty acids with gene expression of omentin in visceral and subcutaneous adipose tissues. Materials and Methods: Visceral and subcut...

Determination of the fatty acid profile of neutral lipids, free fatty acids and phospholipids in human plasma.

BACKGROUND Knowledge of the fatty acid composition of lipid classes in human plasma is an important factor in the investigation of human metabolism. Therefore, a method for the analysis of neutral lipid (NL), phospholipid (PL) and free fatty acids (FFA) in human plasma has been developed and validated. METHODS Separation of lipid classes was carried out by solid phase extraction of the lipid ...